This article has been cited by other articles in PMC. Abstract Chlamydia trachomatis is a very common sexually transmissible infection in both developing and developed countries. A hallmark of C.
Clustering of the strains of Xinjiang, Inner Mongolia and Heilongjiang was confirmed, and this situation was consistent with the close geographical distribution of those provinces.
It is useful for further phylogenetic and epidemiological analyses of Borrelia strains. It is very common and prevalent in temperate climate regions around the world.
This worldwide infectious disease is transmitted to humans by Ixodidae ticks. Since Borrelia burgdorferi B. Up to now, 18 proposed and confirmed species of B.
According to reports, B. Previous study also showed that there were four species in China. As is well-known, different B. And more and more B. In this complicated situation, B.
With the development of molecular biotechnology, there were more and more typing methods which can be used to reflect the phylogenetic or evolutionary relationship for B. However, all of these assays have obvious limitations in identifying B.
Such as single gene loci genotyping shows low resolution power, PFGE genotyping is complicated with low efficiency, the repeatability of RAPD genotyping is poor, and so on.
Simple sequence repeats or variable-number tandem repeats VNTRs are believed to have a high level of discriminatory power. It stems from the significant mutability of the repeat copy number.
Many genomes examined contain numerous VNTRs, and in combination, these can be used to develop a robust PCR-based marker typing system. MLVA has previously shown great discriminatory capacity and accurate estimation of genetic relationships within bacterial pathogens.
Therefore, in this study we focused on the establishment of a new MLVA assay for the genotyping of B. All of the strains were collected from 12 provinces in China. The detail information about the strains was shown in Table 1.
Boiling lysis method was used to get bacterial DNA.GENOVAR BIOSCIENCE LIMITED - Free Company Check: financial information, company documents, company directors and board .
Limitations of Survey-Based Assessments on Duty-Hour Work Standards Richard McDonald, JD, PhD. Author Affiliations.
Richard McDonald, JD, PhD. Senior Principal Scientist Product Development Associate Director GenoVar Biosciences St Clair Shores, Michigan.
Article Information. Medical Education / Graduate Medical Education. Et al.'s work is referenced in diverse journal articles demonstrating technical and research abilities that cross many scientific regardbouddhiste.com al.
does work in AIDS research, cancer discoveries, diabetes, geology, anthropology, astrophysics, and even in research on a worm called C. elegans. How does et al. do it? I could benefit from having et al.
as my next mentor. In fact, just the opposite has been found. Genovar analysis confirms long-held serovar-related arrangements of C.
trachomatis in pathobiology complexes that are unrelated to each other. For example, chlamydial MOMP similarities group a subset of genital phenotypes (D and E serovars) with endemic trachoma phenotypes (B and Ba serovars) and with lymphogranuloma venereum (LGV) serovars (L1 .
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